Physicochemical Properties of Tipula Iridescent Virus

The crystal structure of Tipula iridescent virus as determined by Bragg reflection of visible light.

Crystals of Tipula iridescent virus (TIV)3 diffract light just as ordinary crystals diffract X-rays, so that a crystallographic study is possible using visible light. The conditions of growth of TIV crystals have been investigated and a method developed for preparing large crystals from the microcrystalline centrifuged virus pellets. Optical obsei vations using monochromatic light have been mad...

An electron microscope study of the structure of Tipula iridescent virus.

Localization of Dna and Protein in Tipula Iridescent Virus (tiv) by Enzymatic Digestion and Electron Microscopy

De-embedded ultrathin sections of ethanol-fixed Tipula Iridescent Virus particles were incubated with pepsin at pH 1.8, trypsin at pH 7.7, and DNase at pH 7.7. The outer shell of the particles, but not an inner core, was removed by the action of pepsin. Conversely, the inner core, but not the outer shell, was removed by the action of trypsin and DNase in combination, but not by either enzyme ac...

Genome of invertebrate iridescent virus type 3 (mosquito iridescent virus).

Iridoviruses (IVs) are classified into five genera: Iridovirus and Chloriridovirus, whose members infect invertebrates, and Ranavirus, Lymphocystivirus, and Megalocytivirus, whose members infect vertebrates. Until now, Chloriridovirus was the only IV genus for which a representative and complete genomic sequence was not available. Here, we report the genome sequence and comparative analysis of ...

Fine Structure of the Iridescent Virus Type I Capsid

The structure of the iridescent virus type I was examined using negative staining technique. After storage for r to z months at 4 °C or treatment with chloroform, the icosahedral capsid breaks up into its structural elementspentagons and triangles, each consisting of 31 and 55 subunits respectively. When such a preparation is centrifuged through a linear sucrose gradient (Io to 4o%), 5 zones ar...